Pace Logo

Nucleic Acid Analytical Development

Life Sciences / CDMO/CRO Services / Nucleic Acids / Analytical Development

Analytical Method Development & Testing

Have confidence in your molecule’s safety and efficacy by partnering with our analytical development and testing team. We have experience with nucleic acid therapeutics ranging from small nucleotide drugs and highly-modified oligonucleotides (e.g., ASO, siRNA, PMO/PPMO, PNA) to larger RNA and DNA molecules needed for gene editing and gene therapies. Our experts help you overcome challenges such as molecule size, higher-order structure, and chemical composition/modifications.

Our technical knowledge paired with our understanding of nucleic acid physiochemical properties gives us the expertise to develop methods that meet your specific needs.

Ready To Get Started?
Or Call: 612.656.1175

Molecular Entities

Chemical Synthesis
In Vitro Transcription
Cell Culture
Chemical Synthesis
  • Antisense oligonucleotides (ASO)
  • Short Interfering RNA (siRNA)
  • Conjugated ASO or siRNA (e.g., lipids, GalNAc, peptides, etc.)
  • Phosphorodiamidate morpholino oligomer (PMO)
  • Peptide phosphorodiamidate morpholino Oligomer (PPMO)
  • Guide RNA (gRNA)
In Vitro Transcription
  • Messenger RNA (mRNA) - linear or circular
  • Transfer RNA (tRNA)
  • Guide RNA (gRNA)
Cell Culture
  • Plasmid DNA

Analytical Techniques

  • HPLC and U(H)PLC instruments from multiple manufacturers with a variety of configurations
  • Detectors include UV, ELSD, CAD, fluorescence, mass spectrometers, RI, MALS
  • Mass Spectrometers include high resolution accurate mass (HRAM) with fragmentation (LC-MS/MS)
  • PAGE and Agarose
  • Capillary Electrophoresis including isoelectric focusing
  • UV – variety of cuvette and plate-based systems
  • Fluorescence
    Circular dichroism
Light Scattering
  • DLS, including Zeta Potential
  • MALS
  • Nanoparticle Tracking Analysis (NTA)
Additional Techniques
  • Antibody-based techniques
  • PCR and qPCR-based techniques
  • Asymmetric Field Flow Fractionation (AF4)
  • Karl Fischer
  • ICP-MS
  • GC-MS

Integrated Laboratory Services  

If your project requires additional CDMO support, we offer comprehensive support to advance your nucleic acid. Find more information about how we can help below.

Additional Resources